Exposure to gonadal steroids early in life has lasting (in many cases, permanent) effects on the brain. The nature of this “cellular memory” for hormone exposure has been mysterious but recent work demonstrates that epigenetics (literally, “above” or “on top of” genetics) are involved. Epigenetic modifications of chromatin play crucial roles in the differentiation of cell type by affecting gene expression without changing the underlying DNA code. The two best-understood types of epigenetic marks are the methylation of DNA and covalent modifications of the histone proteins that DNA wraps around (e.g., acetylation or methylation of histone tails). We hypothesized that epigenetic modifications are required for the development of neural sex differences, and have taken two approaches to test this idea: 1) blocking specific epigenetic processes during the critical period for sexually dimorphic brain development; and 2) genome-wide examinations of sex differences in the distribution of epigenetic modifications.
We find that a transient disruption in histone acetylation at birth leads to lasting changes in a well-studied sex difference in the bed nucleus of the stria terminalis (BNST; Murray et al., 2009; McCarthy et al., 2009). Genome-wide examinations of sex differences in epigenetic marks in the BNST were performed in collaboration with the laboratories of Dr. Schahram Akbarian at the Mt. Sinai School of Medicine and Dr. Eric Villain at UCLA. We find sex differences in the distribution of H3K4me3 (the addition of three methyl groups to histone 3 at lysine 4), an epigenetic mark that is often associated with the transcription start sites of active genes (Shen et al., 2015). We also find that neonatal testosterone exposure alters DNA methylation at several hundred genes in the BNST of the mouse brain, and that these effects emerge late, i.e., well after the hormone exposure (Ghahramani et al., 2014). Currently, we are exploring the effects of blocking DNA methylation on sex differences in gene expression in the brain.
To learn about our recent findings, watch this video featured by the Endo Society
Does gender leave an epigenetic imprint on the brain?
Cortes, L.R., Cisternas, C.D., & Forger, N.G. Frontiers in Neuroscience. https://doi.org/10.3389/fnins.2019.00173
Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain
Cisternas, C.D., Cortes, L.R. Bruggerman E.C., et al. Epigenetics. https://doi.org/10.1080/15592294.2019.1649528
Neonatal inhibition of DNA methylation alters cell phenotype in sexually dimorphic regions of the mouse brain
Mosley M., Weathington J., Cortes L.R., et al. Endocrinology. https://doi.org/10.1210/en.2017-00205
Past, present and future of epigenetics in brain sexual differentiation
Forger N.G. J Neuroendocrinol. https://doi.org/10.1111/jne.12492